Immunochemistry-based quantification of tumor-infiltrating lymphocytes and immunoscore as prognostic biomarkers in bladder cancer.

BACKGROUND: Tumor-infiltrating lymphocytes (TILs) and the derived immunoscore (IS) have gained considerable attention over the last decade as prognostic markers in many solid cancers. However, in bladder cancer (BC), their prognostic value is not clearly established. METHODS: The present study aimed to quantify the TILs rates in BC, assess the derived immunoscore, and investigate their prognostic value. An immunochemistry-based quantification of the different subtypes of TILS was performed on paraffin-embedded blocks from patients with invasive urothelial carcinoma of the bladder. We have assessed the rates of TILs, respectively, on peri-tumoral (PT) and intra-tumoral (IT) areas and calculated for each case the corresponding IS which is the index: CD8+/CD3+ TILs. The IS was then classified as low (I0, I1) or high (I2, I3, I4). We included 30 cases in the analysis. RESULTS: The median age of patients was 65 years with a sex ratio of 9. TILs densities and distribution were significantly variable between IT and PT areas CD3+ (p = 0.03) and CD8+ (p = 0.004) with the highest rates on the PT areas. In univariate analysis, a low density of CD8+ TILs was significantly associated with an advanced age (p = 0.05), with the presence of lympho-vascular invasion (p = 0.02) and with the absence of specific histological subtype (p = 0.05). A low immunoscore was significantly associated with the presence of lympho-vascular invasion (p = 0.004). No significant association was found between TILs subpopulations, the IS, and the other clinicopathological and survival data. The overall survival (OS) and disease-free survival (DFS) medians were slightly superior in highly T (CD3+/CD8+)-cell infiltrated tumors as well as tumors with a high IS densities. However, the univariate analysis showed that TILs and immunoscore did not impact overall survival (OS) and disease-free survival (DFS). CONCLUSION: TILs and immunoscore might be effective prognostic tools in BC. However, standardized quantification methods and further investigation on larger samples are highly recommended to definitively attest the prognostic value of TILs and IS in BC.

Pyelonephritis complicated by a perirenal abscess in a pregnant woman: Exceptional cause of fetal death in utero.

Acute pyelonephritis is the most common bacterial infection during pregnancy. If not diagnosed and treated promptly, it can lead to serious maternal and fetal complications. In the literature, a few cases of perirenal abscess complicating acute pyelonephritis during pregnancy have been reported. Herein, we report a case of perirenal abscess in a pregnant woman complicated by intrauterine fetal death and discuss the diagnostic and therapeutic difficulties.

Urothelial carcinoma of the proximal ureter revealed by spontaneous forniceal rupture: A case report.

Spontaneous rupture of the upper urinary tract is a rare and potentially serious pathology. Lithiasis is the most frequent cause. However, other causes are incriminated such as trauma, retroperitoneal fibrosis and especially stenosing tumors. Few cases of excretory tract rupture secondary to tumor obstruction have been reported in the literature. We report a case of spontaneous rupture of renal pelvis secondary to ureteral obstruction caused by urothelial tumor and discusses difficulties of diagnostic and treatment.

Primary urothelial carcinoma of the male anterior urethra; A case report.

Introduction: and importance: Urethral cancer is a rare condition. It represents less than 1% of all cancers and the clinical signs are not very specific and confusing, which explains the often-late diagnosis. The prognosis can be poor. The objective of our observation is to discuss, through a review of the literature, the diagnostic and therapeutic means of tumors of the male anterior urethra. Case presentation: This is a 54-year-old patient, smoker, followed for urinary lithiasis. He initially consulted us for right nephritic colic, with an obstructive iliac ureteral stone on a CT scan. He had drainage by JJ stent followed by extracorporeal lithotripsy. After three months, it was decided to remove the JJ Stent. During urethrocystoscopy, a papillary tumor of the anterior urethra was found, measuring 0.5 cm on the long axis. Endoscopic resection of the tumor was performed. Pathological examination revealed a low-grade malignant urothelial carcinoma. No tumor recurrence was noted after one year of follow-up. Discussion: Primary urethral cancer is a rare condition. Most series in the literature show a male predominance. The main risk factors are chronic inflammation, history of urethritis, and urethral stricture. The clinical signs of urethral cancer are usually discrete and not very specific. The reference examination for positive diagnosis remains urethroscopy, which allows a more precise diagnosis thanks to a direct view of the urethral mucosa. The management of tumors of the anterior urethra consisted of aggressive excisional surgery. The main goal was to achieve satisfactory local control. conservative treatment by resection, fulguration or laser vaporization is indicated for small lesions (<1 cm). Conclusion: Tumors of the anterior urethra in men are often diagnosed late. More effort should be made to diagnose this disease earlier, especially in high-risk groups. Conservative treatment can be offered in patients with a small lesion.

[Correlation between detrusor thickness, intravesical prostatic protrusion and maximum urinary flow in the monitoring of benign prostatic hyperplasia]. / Corrélation entre épaisseur du détrusor, index de protrusion prostatique et débit urinaire maximal dans le suivi de l'hypertrophie bénigne de la prostate.

INTRODUCTION: Detrusor thickness (DT) and intravesical prostatic protrusion (IPP) are closely related to bladder outlet obstruction. The aim of our study was to look for correlation between DT, IPP and maximum urinary flow (Qmax). METHODS: It is a prospective, observational study including men over fifty managed for benign prostatic hyperplasia. Low urinary tract symptoms were assessed with the International Prostatic Symptom Score (IPSS). Pelvic ultrasound was performed for all patients measuring prostatic volume, bladder volume, post- void residual, DT and IPP. Uroflowmetry was performed for all patients, Qmax was noted. Qmax equal or less than 15ml/s was considered pathologic. RESULTS: Sixty patients were included for our study. Strong negative correlation was noted between DT, IPP and Qmax (r=-0.59, r=-0.61 respectively). Patients with pathologic Qmax had higher DT and IPP than those with normal Qmax, the difference was significant (P<0.01). Threshold values predicting pathologic Qmax were 3mm for DT and 7mm for IPP. ROC analysis reveals for DT an AUC of 0.84 (95% CI 0.76-0.92) and for IPP an AUC of 0.88 (95% CI 0.80-0.97). CONCLUSION: Detrusor thickness and intravesical prostatic protrusion have strong negative correlation with Qmax. These parameters could be an alternative to Qmax measurement if uroflowmetry is unavailable. LEVEL OF EVIDENCE: Grade B.

Xeroderma pigmentosum and renal leiomyosarcoma: A very rare case report association.

INTRODUCTION: Xeroderma pigmentosum (XP) is a rare genetic disorder causing extreme sensitivity to ultraviolet (UV) rays. It is transmitted by an autosomal recessive mode. This disease is classically characterized by a high risk of skin cancer. The occurrence of extra-dermatological cancers in patients followed for XP has been rarely reported in the literature. We present the case of a 14-year-old adolescent with an exceptional association of XP and renal leiomyosarcoma. PRESENTATION OF CASE: A 14-year-old adolescent was presented with an abdominal mass revealed by hematuria and abdominal pain. Computed tomography (CT) of the abdomen showed an enhancing, heterogeneous right renal mass, consistent with the features of renal tumor. After non conclusive biopsy, a total nephrectomy of the right kidney was performed. Based on gross specimen observation and immunochemical analysis, the patient was diagnosed with high-grade renal leiomyosarcoma. An adjuvant chemotherapy was indicated and the patient was referred to medical oncology. At 12 months follow-up, the patient is in total remission; The CT scan did not show any locoregional or distant recurrence CONCLUSION: The risk of renal tumor is increased in patients followed for XP. Attending physicians should ask for renal imaging at the slightest sign of clinical call.

Ureteral tumor in an ectopic duplex system: a case report.

INTRODUCTION: Ureteral ectopia is a rarely observed anomaly. It may be totally asymptomatic. An association with a duplex system is exceptional. Diagnostic and therapeutic approaches are challenging. Carcinologic surgery must consider the anatomic variant, mainly related to the ectopic site of the ureteral orifice. OBSERVATION: We report a case of a ureteral urothelial carcinoma in a North African 52-year-old male patient, in a right duplex system. Radiological explorations concluded a non-functional upper right kidney. A suspect mass was observed in the lumbar part of the ureter of the right upper system. The meatus of the tumorous ureter ended in the right lobe of the prostate. A right hemi-nephro-ureterectomy was performed. A histological examination concluded a pT2G2 urothelial carcinoma. CONCLUSION: Even if malignancy is rarely observed in ureteral ectopia, it should be evoked mainly in cases of hematuria with risk factors for urothelial tumors.

Mixed tumor of the kidney.

Mixed epithelial and stromal tumour (MEST) of the kidney, is a recently recognized and rare entity .We herein reported the case of a 56 years old post-menopausal woman who presented with right abdominal pain. Her physical examination was unremarkable. Ultrasonography revealed the presence of a right cystic renal mass in the interpolar region extending into the pelvis. The tumor was considered Bosniak 4 category and a right nephro-ureterectomy was performed. The histological examination of the tumor revealed a mixed tumor with both epithelial and stromal pattern.

[Atypical renal cysts]. / Les kystes atypiques du rein.

BACKGROUND: Atypical renal cysts are suspicious for malignancy. It is hard in some cases to give a sharp radiological diagnosis and propose an appropriate management. AIM: To give the histological results of the operated atypical renal cysts and to establish a correlation between the radiological findings and histological features. METHODS: Between January 2001 and December 2012, 22 patients have been operated in our department for atypical renal cysts. There were 10 type III and 12 type IV Bosniak lesions. We have reported clinical, epidemiological and radiological findings. We have analyzed the histological results taking into consideration the radiological findings. RESULTS: According to histological findings, 7 among the 10 type III lesions were benign (70%). However, only one case of benign tumor was noted in type IV lesions (8.3%). There was no evident correlation between tumors size and histology. With a median follow up of 48 months, three patients died because of locally advanced tumors. CONCLUSION: Management of atypical renal cysts remains difficult. A careful computed tomography staging is essential before treatment. Type III and type IV Bosniak cysts were malign in 30% and 91% of cases, respectively. There was no correlation between tumor size and malignancy. A nephron sparing surgery should be proposed whenever possible.

[Endoscopic realignment versus delayed urethroplasty in the management of post traumatic urethral disruption : report of 30 cases]. / Le réalignement endoscopique versus urétrorraphie terminoterminale tardive dans le traitement des ruptures post-traumatiques de l'urètre : Notre expérience à propos de 30 cas.

BACKGROUND: Post traumatic posterior urethral disruption is a common condition, its treatment is controversial. AIM: To assess the long term results of endoscopic realignment compared with delayed urethroplasty, in the management of post traumatic urethral disruption. METHODS: Between February 2002 and March 2009, 30 patients have been operated for post traumatic posterior urethral disruption. 20 have had a primary endoscopic realignment and 10 have had delayed urethroplasty. Analysis of the results took into consideration, the quality of micturition, the continence and the erectile function. results: Median follow-up was 21 months, good results were recorded in 13 patients among the group of realignment (65%). 0f the failure cases (7), 3 underwent urethroplasty and 4 were managed by endoscopic treatment. Endoscopic realignment could avoid open surgery in 17 patients (85%). Among the 10 patients that underwent urethroplasty, 7 patients had good results (70%). No patient had urinary incontinence. A post operative erectile dysfunction was noted in one patient from the realignment group and two other patients from the urethroplasty group. CONCLUSION: The endoscopic urethral realignment could be used as a primary therapeutic management of post traumatic urethral disruption. It could be recommended for recent trauma, between one and two weeks, and for patients that can support exaggerated lithotomy position. Endoscopic realignment is an effective safe therapeutic mean that does not contraindicate a second-line urethroplasty.

Renal angiomyolipoma with Fatty thrombus extending to the right atrium: an exceptional presentation.

This paper reports the case of 34-year-old woman who presented with bilateral renal angiomyolipomas (AMLs). On the right side, there was a large AML with a fatty thrombus extending to the right atrium. The treatment consisted of right nephrectomy and complete thrombectomy with extracorporeal circulation and right atriotomy. Postoperatively, the patient was septic and died on postoperative day 7 because of septic shock.

Paratesticular pleomorphic rhabdomyosarcoma: a report of two cases.

Pleomorphic rhabdomyosarcoma (RMS) is a rare tumor with an aggressive behavior, described mainly in adulthood. Herein we present two cases of paratesticular pleomorphic RMS in 71- and 16-year-old patients with metastases at initial diagnosis. Histological, immunohistochemical, and ultrastructural findings were essential to confirm diagnosis. Few months after radical orchiectomy, both patients died before or just after starting adjuvant chemotherapy.

A colourachal-cutaneous fistula: a report of an unusual case.

Urachal anomalies are rare affections due to incomplete closure of urachus during foetal period. Colo-urachal-cutaneous fistula is an uncommon complication of urachal anomalies. Only three cases have been reported so far in the literature. Herein, we report a new case in a 51-year-old patient presenting with umbilical feculent discharge lasting for 4 weeks. Diagnosis was made by computed tomography. After en bloc total surgical excision followup was uneventful.

The long-term results of laparoscopic retroperitoneal pyeloplasty in adults.

OBJECTIVES: To report the long-term outcome of laparoscopic retroperitoneal pyeloplasty (LRP) in adults. PATIENTS AND METHODS: Thirty patients underwent LRP for primary pelvi-ureteric junction obstruction (PUJO). Anderson-Hynes dismembered pyeloplasty was used in 28 patients and a Foley Y-V pyeloplasty in two. A JJ stent was inserted antegradely during the procedure. Patients were reviewed at 1 month after LRP for stent removal, and then at 6 and 12 months routinely, using excretory urography. RESULTS: The mean patient age was 29.7 years, with a female predominance of 60%. Conversion to open surgery was mandated by dense adhesions secondary to previous pyelonephritis in three patients, and difficulty in suturing in one. The mean (range) operative duration was 228 (190-280) min. There was a crossing vessel in 11 patients and it was not transposed in any. The mean hospital stay after LRP was 4.2 days. The mean (range) follow-up was 60 (29-106) months. Of the 26 patients who had complete laparoscopic procedures, 23 had no evidence of obstruction on long-term postoperative intravenous urography and/or diuretic renography. CONCLUSION: LRP combines the high functional success rate of open pyeloplasty in the long term and the minimally invasive morbidity of laparoscopy.